A single script! What it does: demultiplexing of metabarcoding data which consists of multiple markers.
- Data must have followed a library preparation/sequencing strategy which includes sequencing of the forward primers.
- Data must be demultiplexed for samples already.
- Sequence data must be in forward orientation.
The categorization is based on Hidden Markov Model (HHMs) hits of the forward primer within the first 20 bp. This is very fast, and allows high throughput of the data.